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WELCOME TO THE BIOCHEMISTRY LABORATORY! This Biochemistry laboratory seeks to model work performed in a biochemical research laboratory. The course will guide you through basic lab techniques, investigations into DNA and enzyme kinetics, an intensive purification and characterization of an unreported protein, and will culminate in a formal research paper in the format of an article published in Biochemistry. Module 1 is concerned with basic lab skills. In these labs, we will learn how scientists think and write about biochemistry and perform experiments. We will also learn to accurately and precisely measure small volume of liquid while avoiding sample contamination. Lastly, we will learn to compute and create buffer solutions—a cornerstone of biochemistry. Module 2 will allow us to purify the protein cytochrome c from a yeast species (Saccharomyces cerevisiae) using various fractionation techniques including homogenization, centrifugation, and column chromatography. We will characterize our products using biochemical methods including gel electrophoresis, UV-Vis spectroscopy, and electrochemistry. Using modeling software on the computer, the structure and function of model, comparison cytochrome c proteins will be investigated. As a result of this project, we will determine the molecular weight, the approximate number and type of aromatic residues, characteristic UV-Vis spectra, and denaturation/renaturation properties of cyctochrome c. Module 3 looks into the processes used to isolate, purify, amplify, and characterize DNA. We will isolate and purify DNA from a bacterial source, and design and then use then use the polymerase chain reaction (PCR) to amplify a DNA region of interest to ascertain the nature of the DNA we purified. Finally, we will perform in silico studies of DNA cloning, followed by DNA restriction and ligation for transformation into a bacterial expression system—molecular cloning. Module 4 is focused on enzyme kinetics, the measurement of the extent and mechanism by which enzymes catalyze biological reactions. We will investigate these processes by looking at the activity of tyrosinase found in mushrooms, which catalyze oxidation of various substrates. We will also investigate the effect of enzyme inhibitors of these reactions. The emphasis of the lab is on learning to perform complex biochemical techniques, as well as on analyzing and interpreting data and using graphing programs. Lab instructions and report expectations are explained in the pages that follow.
This manual was prepared by the collaborative efforts of the University of Virginia Chemistry Department graduate and undergraduate students. Lauren Lee and Ana Wang developed and tested many of the protocols so that each one could be efficiently conducted in an undergraduate teaching laboratory. Daniel Fox, Ling Huang, Tomasz Kabsinski, Brett Kroncke, Jenny Lounsbury, William Peairs, and Brian Poe prepared this manual and improved upon the protocols to enable the students to obtain meaningful results. In addition, these students worked together to transform the biochemistry laboratories into a productive and fun space. CHEM4411 Biological Chemistry Lab I This course is designed to give you a glimpse of the general methods utilized in a biochemistry laboratory. You will perform techniques such as chromatography, PCR, SDS-PAGE gel electrophoresis, and many more. The lecture each week will address the method, data, and interpretation of the results for each week. The answers will not be given to you. You will need to perform literature searches and dig for relevant data in the literature to understand and compare to your data. This is all meant to provide you with the tools to conduct research, both in and out of the lab. Required materials Lab Manual: Available at the bookstore. Lab Notebook: Buy the type with carbon capabilities and duplicate numbered pages. Text: Fundamental Laboratory Approaches for Biochemistry and Biotechnology by Ninfa, Ballou, and Benore. Comments about Biochemistry Laboratory Protocols All of these experiments work. The results may not be what you expect, and interpretation of your data is not necessarily straightforward. If you don’t obtain good results, there are sample data available that have been obtained by the protocols provided to you. If you need to use the sample data, then you need to discuss what you did wrong and what could be improved. It is not enough to just do the protocol given to you. You must understand why you are doing a particular procedure and what the purpose of each step is. There are particular labs that require you to come in the evening before and the morning of your laboratory. Plan ahead...
This document is meant as a guide to developing applications for real-time audio processing and playback in iOS, using Core Audio services. This guide presents a broad overview of audio in iOS, as well as speciﬁc details regarding the implementation of various audio programming strategies. Important terms to be familiar with are shown in bold and deﬁned in the glossary at the back of this document. Code samples and names of ﬁles containing code will be shown in this italicized font Code is taken from Appleʼs sample iOS applications as well as the iZotope audio effect sample application, distributed with each iZotope iOS SDK. Who is this guide for? This guide is meant to be a resource for developers who are new to Core Audio on iOS. It will be most useful to programmers who are experienced in iOS development but not in audio technology, or to audio programmers who are not experienced in iOS development. As such, it is highly recommended the reader have an understanding of basic concepts of both iOS development and digital signal processing before consulting this guide. For more information on DSP, or iOS development and audio programming, take a look at the Appendix - Introductory DSP Tutorial and Additional Help and Resources sections of this guide to ﬁnd useful information, as well as links to other resources on the subject.
HUMAN INTERVENTION An example of human intervention is a person fills out a form about a problem they’re having and clicks submit. The process is kicked off by a person’s explicit action, but the person who causes the process to start may not be in the BPM environment. They could be in a portal environment or some other environment that hides the nature of the BPM environment from them. SCANNED DOCUMENT Another common way to initiate a process is to have a document arrive in a content management system, and the document requires some sort of process in order to be executed. There are still a lot of processes that are driven by the arrival and scanning of paper documents, but this could be any kind of content that’s added to a content repository. It could be a Word document; it could be an email. Whatever it is, you have a piece of content, you add it to a content repository (for records management or other retention purposes), and there’s typically some sort of process that needs to happen around that document. It could be to gather information from that document, such as keying in transactional data from a scanned application form. It could be a review and approval cycle for an electronic document like a press release. In both cases, the creating and adding of that content to a content repository kicks off a process. EXTERNAL EVENT We can also have external events trigger processes, typically a transaction or an alert from another system. One example of this is a mainframe insurance underwriting application, where it does the underwriting automatically in the mainframe system, but if something goes wrong, and a person needs to look at a particular instance of a process, the mainframe process triggers a process in a BPM system to have someone take a look and resolve the problem. INVOKED AS WEB SERVICE Although external events will often trigger a process by invoking BPM as a web service, I include the invoking of the web service as another way to kick off a process to cover any case where you have a separate application or another...
CCARD Ltd is an independent consultancy offering CAE hardware and software installation and customisation services, specialising in CATIA and I-DEAS systems. CCARD also specialises in Electronic Data Interchange (EDI), and supplies, installs and supports OFTP/Odette based ISDN or TCP/IP solutions. In order to continue to provide its customers with the best products and support, CCARD has negotiated exclusive access to a CATIA V5 Introduction User Guide, of unique quality and effectiveness, which is ideal as a cost-effective self-study tutorial. CCARD can be contacted either by telephone on 024-76-226888 by emailing email@example.com or via our website at www.ccard.co.uk This extract from the CATIA V5 Introduction User Guide Includes the contents and index pages, together with the full initial worked example, overviews and summary of all examples, of the complete 134 page spirally bound manual. Provides an illustration of the style and content of this and other CATIA V5 User Guides compiled and published by The CAD/CAM Partnership - the leading independent CATIA specialist in the UK. Assumes the availability of a CATIA V5 workstation with a configuration license (such as ‘MD2’) and also familiarity with the CATIA V5 interface, such as use of the mouse buttons and command icons, in order to follow the initial worked example provided as an isolated sample.
This consumer-facing website offers many ways to inspire organizational solutions in beautiful cabinetry options, as well as drive business to our dealers. Here are some highlights: • View inspirational designs and photos. • Download floor plan drawings from photosets to gather design ideas. • Visualize door styles by wood species and finish. • Use the “Features & Accessories for Your Lifestyle” to view organizational solutions by room type. • Engage consumers by directing them to the “Step-by-Step Kitchen Planner” to create a mood board to share with their designer. • Educate consumers about wood characteristics, installation, Merillat sustainability initiatives, and dos and don’ts of cabinetry care. MerillatBusiness.com If you’re not currently registered for MerillatBusiness.com, you could be missing important product and brand information that can assist you with your Merillat sales efforts. It’s quick and easy, so visit the site and register today. It will enable you to: • Check order status. • Download or order product literature, like the product brochure, specification and price books, door/style finish codes, and 20-20 Design updates. (For questions on 20-20 Design, email firstname.lastname@example.org.) • Download promotional materials, such as brand campaign print ads, direct mail postcards, videos and counter cards. • Order samples and displays, like wood chips, selection centers, and sample doors and backer cards. Merillat Customer Care Resources Electronic means are convenient, but sometimes you just need to talk to a person, and Merillat Customer Care consultants are here to help. Your consultant can help you: • Order product. • Follow up on orders or track delivery progress. • Discuss product situations or address invoice questions. Your Merillat® Account Manager is there to help you … every step of the way. Make sure to contact them whenever you have questions, concerns or need information. Additional assistance is available through Customer Care: (855) 896-9803, (855) 896-9798, or email@example.com For NEW ORDERS ONLY: (866) 998-4432, or firstname.lastname@example.org For Quality Assurance: email@example.com
AHRI White Paper: Reports of R-134a Contaminated with R-40 and Other Refrigerants Counterfeit R-134a, containing R-40 (Methyl Chloride or Chloromethane), has been found in mobile air conditioners, stationary air conditioning, and transport refrigeration systems in many countries. For your personal safety and to avoid serious injury or death, special care must be taken when accessing the service ports to sample or work on systems that are not functioning properly or have been serviced by others. R-40 chemically reacts with aluminum inside HVACR systems to generate highly reactive and/or toxic compounds, and exposure of the system’s contents to air and/or moisture could result in production of a strong acid and violent chemical reaction. Several container systems have exploded at service facilities, some resulting in fatalities. Some of these systems were found to contain R-40 and/or other unacceptable substances. Compounds such as R-40, R-22, R-142b, R-152a, and R-12 have been found mixed with R-134a in newly filled refrigerant cylinders marked as containing R-134a. There have also been instances of counterfeited brand name R-134a cylinders sold containing refrigerants other than R-134a. Research is being conducted by ASHRAE to identify the reaction mechanisms for R-40 and aluminum inside systems, as well as other reaction products. It is also necessary to determine the concentration of R-40 that is safe as a contaminant. AHRI Standard 700-2012, Specification for Fluorocarbon Refrigerants, would allow up to 0.5% of other volatile impurities. There have been no known issues arising from refrigerant that meets AHRI standard 700. Unfortunately, the problem with counterfeit and contaminated refrigerants goes beyond just R-134a. Other refrigerants, including R-22, R-404A, and R-410A, have also been found to be counterfeit and in some instances badly contaminated. Cylinder labels and packaging have been counterfeited in some instances, and therefore are not reliable indicators of the authenticity of the contents of a cylinder. Contaminated refrigerants can cause a variety of issues that may range from increasing energy use and decreasing cooling performance, to significantly reducing the operating life of equipment, and even dramatic and injurious equipment failures, as in the case of R-40 contamination. In addition, some counterfeit refrigerant products contain varying amounts of ozone-depleting substances, which may be illegal in certain parts of the world under the terms of the Montreal Protocol. It is good practice to purchase refrigerants from reputable, reliable sources. Failure to do so could result in the purchase of counterfeit refrigerants that contain substances such as R-40...
General Information Please note: • The Race Kit parts detailed in this publication are made in accordance with FIM technical regulations and are NOT road legal. • The Race Kit parts covered in this publication are intended for racing purposes only and any Triumph motorcycle fitted with such kits MUST NOT be used on public roads. • The Race Kit parts detailed in this publication may only be used on a closed-circuit in the hands of experienced riders. • Before fitting any Race Kit parts, customers should check the technical regulations of their race class to ensure conformity. • The information provided in this publication should always be used together with the official Triumph Daytona 675 service manual. • Completely read all the instructions before commencing the installation and set up of the Race Kit in order to become thoroughly familiar with the kit’s features and the installation process. • When removing components which incorporate a gasket ALWAYS ensure a new gasket is fitted on re-assembly. • The Race Kit parts detailed in this publication are not covered by any warranty. • Specifications are subject to change without notice. • The information contained in this publication is accurate at the time of final approval, however, Triumph Motorcycles LTD reserves the right to amend the information at any time without notice. • Whilst every effort is made to include the latest information in the service manual, this is not always possible. The latest information and technical changes are provided to authorised Triumph dealers via Technical News. It is recommended you contact an authorised Triumph dealer to request this information. Warnings, Cautions and Notes Throughout this publication particularly important information is presented in the following form: Warning This warning symbol identifies special instructions or procedures, which if not correctly followed could result in personal injury, or loss of life. Caution This caution symbol identifies special instructions or procedures, which if not strictly observed, could result in damage to, or destruction of, equipment. Note: • This note symbol indicates points of particular interest for more efficient and convenient operation.
Lesson 2 Mass Elements Mass Elements are used to give you a conceptual idea of the space and shape of a building without having to take the time to put in a lot of detail. It allows you to create alternative designs quickly and easily and get approval before you put in a lot of effort. Massing Tools Creates a solid shape. Controls the visibility of mass objects. Inserts a mass group into the active project. Model by Face: Converts a face into a Roof, Curtain Wall System, Wall, or Floor. When creating a conceptual mass to be used in a project, follow these steps: 1. Create a sketch of the desired shape(s). 2. Create levels to control the height of the shapes. 3. Create reference planes to control the width and depth of the shapes. 4. Draw a sketch of the profile of the shape. 5. Use the Massing tools to create the shape. Masses can be used to create a component that will be used in a project - such as a column, casework, or lighting fixture - or they can be used to create a conceptual building. Shapes Drawing Name: Estimated Time: shapes.rfa 5 minutes This exercise reinforces the following skills: •Creating the basic shapes using massing tools •Create an extrude •Modify the Extrude height •Create a revolve •Create a sweep •Create a blend •Modify a blend 1. There are several sketches in the file. Each set of sketches will be used to create a specific type of mass form. 2. The most basic mass form is an Extrude. This mass form requires a single closed polygonal sketch. The sketch should have no gaps or selfintersecting lines. Select the rectangle so it highlights. 3. When the sketch is selected, you will see grips activated at the vertices.
When you get your auto insurance policy, the top page is normally the declarations page, or “dec page.” The dec page shows your coverages and the premiums you must pay for them. Not all companies will send you a new policy every year — you may get only a new dec page. The sample shown here will give you an idea of how the dec page might look. Part A - Liability: Pays when you cause injuries and property damage suffered by others, see page 5. Part B - MedPay (Medical Payments): Pays the medical bills for you and your passengers, see page 6. Part C - UM/UIM (Uninsured/ Underinsured Motorist): Pays when you are injured by a driver who has no or little insurance, see page 9. Part D - Damage to Your Auto: Pays damages to your vehicle whether or not caused by a collision, see page 10. Personal Auto Policy Declarations Policyholder: Your name and address Policy Number: Your policy number Policy Period: From: Start date o: T End date (often six months from start date) But only if the required premium for this period has been paid, and for six-month renewal periods if renewal premiums are paid as required. Each period begins and ends at 12:01 A.M. standard time at the address of the policyholder. Insured vehicle and schedule of coverages Vehicle Coverages