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MEMBRANE AND CELLULAR BIOCHEMISTRY MBIO 3460 LAB ...

CONTENTS PAGE ______________________________________________________________________ EXPERIMENT SCHEDULE 3 GENERAL INSTRUCTIONS 4 LAB STANDARD OPERATIONS PROCEDURE 9 WHMIS 12 1 THE ISOLATION AND QUANTITATION OF UBIQUINONE Saponification Extraction and Evaporation TLC Scanning spectrophotometry Lab Report Do you know? Reagents and Functions 15 2 THE ISOLATION OF RED BLOOD CELL MEMBRANE PROTEINS Preparation of Red Blood Cell Membranes Protein Determination Polyacrylamide gel electrophoresis Lab Report Do you know? Reagents and Functions References 31 APPENDIX Excel Procedures 46 Pipetman Operation 49 Hitachi High Speed Himac Refrigerated Centrifuge 50 Sorvall Legend X1R Centrifuge Operation 52 Biochrom Ultraspec pro500 Operation 54 Biochrom Spec3100 Operation 55 SAMPLE LAB EXAM 56 3 MBOI 3460 Membrane and Cellular Biochemistry SCHEDULE 2013 All labs start at 2:30 pm unless otherwise stated. Section assignment is based on last name - see lab website for details. Week Section Room # Experiment/In-lab Assignment Date # 1 1 204 The isolation and quantitation of ubiquinone (1) Saponification Sept 13 2 2 204 The isolation and quantitation of ubiquinone (1) Saponification Sept 20 3 1 2 204 201 The isolation and quantitation of ubiquinone (2) TLC Sept 27 4 1 204 The isolation and quantitation of ubiquinone (3) Scanning spectrophotometry Oct 4 5 2 204 The isolation and quantitation of ubiquinone (3) Scanning spectrophotometry Oct 11 6 1 2 204 201 The isolation of red blood cell membrane proteins (1) Isolation and Bradford protein determination Oct 18 7 1 2 204 201 The isolation of red blood cell membrane proteins (2) Lowry protein determination and Polyacrylamide Gel Electrophoresis Oct 25 8 1&2 534 Nov 1 11 1&2 TBA EM Microscope Demonstration, 534 Buller, wait outside door at designated time for your group: groups 1 - 8 attend at 2:30 pm; groups 9-16 attend at 2:50 pm; groups 17-24 attend at 3:10 pm Lab exam Nov 22 MBIO 3460 Report Due Dates Week # Description Datea ....

“If Looks Could Kill” Toyota - Amazon Web Services

Business Situation and Campaign Objectives Background and Business Objectives Within the African-American consumer market (AACM), the midsize passenger car segment is a fierce battleground. This category includes the best-selling Toyota Camry as well as two wellknown rivals – Nissan Altima and Honda Accord. The launch of the redesigned 2007 Camry was seen as an opportunity to steal share from the competition but the buyers Toyota hoped to steal did not see a reason to test drive the new Camry. Before even seeing it, African American buyers who were interested in Altima and Accord had stereotyped Camry as a “boring car for boring people”. The challenge was to convey that the new Camry was the opposite of “boring”; to position Camry as just as surprising and unexpected as the buyers themselves. Marketing Strategy The marketing objectives were to: Generate awareness for the Camry’s styling and technological advancements. Change perceptions of Camry among African-Americans intending to buy competitive brands (“conquest intenders”) by delivering a surprising experience that would cause the target to reconsider Camry (Increase consideration). Budget The total budget for the assignment (“If Looks Could Kill” campaign) was approximately $2-5 million, spread out over media, production and promotion/PR.

Getting Started - Support - Panasonic
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Example Network Diagram ..................................................................................... 6 Network Devices and Numbering Plan .................................................................. 7 Network Application................................................................................................... 8 Numbering Plan Example.......................................................................................... 8 Numbering Plan Summary ...................................................................................... 12 Preparations........................................................................................................... 26 Preparing the PC ..................................................................................................... 26 Programming the VoIP Gateway Card in the Los Angeles Office ..................... 29 Starting the IP-GW4 Maintenance Utility ................................................................. 29 Changing the Status of the VoIP Gateway Card...................................................... 31 Assigning the IP Address ........................................................................................ 32 Assigning the Hunt Pattern ...................................................................................... 33 Programming the Address Translation Table ........................................................... 34 Downloading the Address Translation Table from the VoIP Gateway Card ............. 37 Rebooting the VoIP Gateway Card .......................................................................... 38 Confirming the IP Address Assignment................................................................... 39 Programming the VoIP Gateway Card in the Chicago Office ............................ 40 Programming the PBX ..................................................................45 Programming the PBX in the Los Angeles Office .............................................. 46 Programming the PBX in the Chicago Office...................................................... 48 A Guidance for VoIP Installation......................................................51

Sample Curriculum Vitae (International with Photo)

CURRICULUM VITAE Personal Information: Surname Given Name: Address: Date of Birth: Place of Birth: Nationality: Martial Status: Schnitzer Wilhelm 99, Xia Xi Shun Cheng Street Chengdu 610016 P.R.China Tel. No. 0086 13708054946 E-mail: zhuschnitzer@hotmail.com July, 12, 1951 Friedingen German Present Resident in China Married with one child (01/11) Objective: To secure a rewarding assignment to lead a 4 or 5 star hotel to its next level through skillful management and by motivating, training and supporting the team to achieve higher standards of performance. Key Skills Culinary background, motivation, organization and leadership Vocational Experience: December.2003 – present General Manager Yinhe Dynasty Hotel Chengdu China A four star property with 364 rooms and 6 F&B outlets, with banquet facilities up to 1200 person. In 2005 we generate a turnover of $6.1m. During my tenure GOP has seen a sharp increase from 16.06% in 2003 to 22.38% in 2005, an increase of 39.35%. The workforce consists of a total of 460 associates

How to Buy a Replacement Dell Latitude E6410 Battery and Adapter    www.notebookbatteries.co.nz

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Passing Score for Each Section of the Certified Paralegal Examination

Passing Score for Each Section of the Certified Paralegal Examination The Certified Paralegal examination consists of five sections: Communications, Ethics, Legal Research, Substantive Law, and Judgment and Analytical Ability. All five sections must be passed to achieve the Certified Paralegal credential. The Certifying Board determines the passing score for each section based on a method entitled the Angoff Method. This method is widely used by certification programs to set standards. This process requires that a passing score is established by a panel of content experts based on their review of each examination question and assignment of an expectation of the percentage of minimally-competent qualified examinees that would answer each question correctly. Panel members include paralegals, attorneys, and paralegal educators. The procedure for review requires several steps. The steps are:...

Sistem Aplikasi Laporan Bencana
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1. Klik pada Login Staff 2. Masukkan ID dan kata Laluan 3. Jika Berjaya, Klik pada button OK 4. Klik pada Button Laporan Bencana 5. Klik Pada Sistem Aplikasi di sebelah kiri 6. Klik Pada Laporan Bencana di sebelah kiri 7. Klik Laporan Bencana di sebelah kanan 8. Isikan maklumat utama seperti dibawah. 9. Klik OK 10. Isikan tarikh kejadian dan masa berlaku bencana. Untuk pengisian lokasi berlakunya bencana, Koordinat GPS diperlukan, klik butang RUJUK. 11. Paparan berikut akan dipaparkan, Klik pada Icon dan heret ke lokasi kejadian, contohnya di Pulau Pinang. 12. Setelah anda mengenalpasti lokasi koordinat bencana, Tekan butang Salin. ** TIPS : Klik Zoom ataupun pilih imej Satellite untuk paparan yang lebih jelas. 13. Jika ada laluan alternatif, tekan butang RUJUK.

Consumer Products Packaging Design - Wright Design

PConsumer Products Packaging Design The Design Process and Some Basic Tips Package design for a new consumer product is as important as the product inside. This article will present a brief roadmap for designing the package for optimum effectiveness. Ideally the package designer will be a professional with strong consumer products experience. Except for the product itself, there is nothing more important to a product’s success than having a hardworking, truly sales generating package. If the package is not designed right, the trade will never buy the product and the consumer will never try it. The first section of this article reviews a typical Design Process. The second section offers some basic Packaging Design Tips. The Design Process Packaging Design - Preparation Here’s a typical Design Process. It is important to use a step by step organized approach to develop packaging design. Among other things, it helps to define which steps are client responsibilities and which are responsibilities of the design firm. 1. The first step in the process is for the client to describe the assignment to the design firm, assemble all relevant information and materials, list product benefits and key points in their priority of importance...

AOneAssignment.com, an Essay Writing Company provide online assignment help!

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METRO-NORTH RAILROAD CAREER OPPORTUNITIES

Summer Internship Opportunities Multiple opportunities are available. Work location will vary per position. Positions will be launched over the next 4-5 weeks and will be open until filled.  12 week program runs from 5/28-8/16.  Undergraduate pay rates are $15 per hour. Graduate and Law student pay rates are $17 per hour.  Free commuting on Metro-North Railroad.  Working knowledge of Microsoft Office Suite (Word, PowerPoint, Excel).  Position may require limited travel to our various New York and Connecticut locations.  To qualify for credit earning internships applicants must obtain approval from your Student Career Placement Office.  Good oral and written communication skills.  Good analytical and organization skills.  Excellent interpersonal and customer service skills to include dealing with the general public.  Flexibility to work as a team member, supportive of all Metro-North Railroad initiatives.  Must be dependable and a self-starter.  Monthly journal entries and final project assignment will be required as part of the Internship program.  A background check is required.  A physical abilities test and medical exam will be required for selected candidates at our NYC facility.  Students will be required to attend New Hire Orientation and Speaker Series meetings in our NYC facility.  All Interns are required to complete a Final Group Project and presentation. PREFERRED EDUCATION/EXPERIENCE Applicants must be a matriculating Undergraduate, Graduate, or Law student with a GPA of 2.5 or higher and a focus in the following fields:  Accounting  Communication/Journalism  Economics  Engineering (Civil, Mechanical, Electrical, Structural, Computer, Industrial)  Environmental studies  Finance  Hotel/Hospitality Management  Human Resources / Training/ Organizational Behavior / Industrial / Organizational Psychology  Information Technology/Computer Science ...

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