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Optimization the production of extracellular lipase in culture of Bacillus cereus has been investigated. From 10 samples of diesel fuel polluted soil it was found that 3 samples was positive for the presence of B. cereus. Lipase production by B. cereus isolated from diesel fuel polluted soil was investigated and optimized. The enzyme production was increased with increasing time and maximum enzyme activity was obtained after 72 hrs of incubation. Effect of pH and temperature indicated that, the lipase production was maximum at pH 8.0 (60.2 U/ml) and at 35°C (55.25 U/ml). The lipase production was optimized in shake flask experiments. With a selected carbon sources, maltose (65.5 U/mL) and nitrogen source, peptone (66.25 U/mL) was suitable substrates for accelerating lipase production. The present study indicates that the various factors influenced enzyme production by the bacteria, it appears that the nature of the supplements had significantly influenced on the produc
Burkholderia cepacia is a gram negative bacilli usually isolated from persons with genetic lung diseases such as Cystic fibrosis. It was discovered as a plant pathogen but later researchers have found it to be a notorious pathogen in humans. It is also a nosocomial pathogen which contaminates equipment and even disinfectants. In the other end of the spectrum, research suggests that these organisms maybe used as bio diesel and also as pesticides and fertilizers. This review of literature gives an idea of the organism and the gateway it opens for further research with a special note on the evolution of this genus as a nosocomial infection in Neonates.
Biodiesel refers to a vegetable oil- or animal fat-based diesel fuel consisting of long chain alkyl (methyl, propyl or ethyl) esters. Biodiesel is typically made by chemically reacting lipids with an alcohol. Biodiesel is meant to be used in standard diesel engines and is thus distinct from the vegetable oils. Biodiesel can be used alone, or blended with diesel. Biodiesel, a promising substitute as an alternative fuel has gained significant attention due to the predicted shortness of conventional fuels and environmental concern. The utilization of liquid fuels such as biodiesel produced from Jatropha oil by transesterification process represents one of the most promising options for the use of conventional fossil fuels.
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Take EXIT 19B Needham Bear right off ramp onto HIGHLAND AVE At MUZI FORD, Right turn onto GOULD STREET Gould Street ends at CENTRAL AVE Right turn onto CENTRAL AVE Third right onto RESERVOIR STREET On Reservoir Street, RIVER BEND OFFICE PARK will be on your left. Continue on Reservoir Street, passing the first entrance into the park. Take LEFT at the last entrance into the park. Continue down the DRIVEWAY to the last office on the LEFT, SUITE 21 with a WHITE PORCH.
My complaints started beginning year three the unit gave a dirty oil sample; this was questioned but disregarded by mechanics as an oversensitive sample machine. Truck then blew both turbos; this was covered under warranty and diagnosed as the culprit for oil sample. Three days later truck began to smoke considerably, brought unit back in and told engine was dusted due to a faulty CGI pipe which was a freightliner part not covered by their engine or exhaust warranty. The pipe had a hole and had taken in dirt which first caused turbo failure and the engine failure.