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Stem cell therapy in a caprine model of osteoarthritis - Wiley Online ...

Stem Cell Therapy in a Caprine Model of Osteoarthritis J. Mary Murphy,1 David J. Fink,1 Ernst B. Hunziker,2 and Frank P. Barry1 Objective. To explore the role that implanted mesenchymal stem cells may play in tissue repair or regeneration of the injured joint, by delivery of an autologous preparation of stem cells to caprine knee joints following induction of osteoarthritis (OA). Methods. Adult stem cells were isolated from caprine bone marrow, expanded in culture, and transduced to express green fluorescent protein. OA was induced unilaterally in the knee joint of donor animals by complete excision of the medial meniscus and resection of the anterior cruciate ligament. After 6 weeks, a single dose of 10 million autologous cells suspended in a dilute solution of sodium hyaluronan was delivered to the injured knee by direct intraarticular injection. Control animals received sodium hyaluronan alone. Results. In cell-treated joints, there was evidence of marked regeneration of the medial meniscus, and implanted cells were detected in the newly formed tissue. Degeneration of the articular cartilage, osteophytic remodeling, and subchondral sclerosis were reduced in cell-treated joints compared with joints treated with vehicle alone without cells. There was no evidence of repair of the ligament in any of the joints. Conclusion. Local delivery of adult mesenchymal stem cells to injured joints stimulates regeneration of meniscal tissue and retards the progressive destruction normally seen in this model of OA.

SMART SOLUTION Tes Potensi Akademik SBMPTN 2013

Rangkuman Materi SBMPTN 2013 SELEKSI BERSAMA MASUK PERGURUAN TINGGI NEGERI Disertai Teori Ringkas dan Pembahasan Soal Tes Potensi Akademik (TPA) Disusun Oleh : Pak Anang Kumpulan SMART SOLUTION dan TRIK SUPERKILAT Ringkasan Materi SBMPTN Tes Potensi Akademik (TPA) Penalaran Verbal (Sinonim, Antonim, dan Analogi) By Pak Anang (http://pak-anang.blogspot.com) A. PENALARAN VERBAL 1. SINONIM (Padanan Kata) Soal-soal sinonim, kemampuan yang dituntut adalah adik-adik mampu mencari arti dari sebuah kata pada pilihan jawaban yang tersedia. Tips agar adik-adik mudah menyelesaikan soal tentang sinonim adalah sering membaca. Nah, saat menemukan kata-kata asing, jangan ditinggalkan begitu saja, catat dan coba mencari padanan katanya di Kamus Besar Bahasa Indonesia. TRIK SUPERKILAT: Seringkali ada pilihan jawaban yang hampir mirip dengan soal. Biasanya jawaban ini adalah diberikan sebagai jawaban jebakan. Contoh soal sinonim: PARTIKELIR = .... A. Tukang parkir B. Partisan C. Partisi D. Swasta E. Enterprener Pembahasan: Jawaban B dan C mirip dengan kata yang digunakan pada soal. Biasanya ini mudah kita eliminasi sebagai jebakan jawaban..... Sehingga mempemudah kita dalam menjawab soal sinonim ini. Jawaban yang tepat adalah ”swasta”. 2. ANTONIM (Lawan Kata) Soal tentang antonim ini kebalikan dari sinonim. Dalam soal antonim adik-adik dituntut untuk mencari lawan kata dari soal yang diberikan. Contoh soal antonim: TERKATUNG A.Melayang B.Pasti C.Ombak D.Terperosok E.Terbenam

Carrot Cake - U Weight Loss Clinics

INGREDIENTS Whole wheat flour ¾ cup scoops U-Protein 4½ Baking powder 1 ½ tsp Stevia 13 gm Salt ½ tsp Cinnamon (to taste) 1 ½ tsp Nutmeg (to taste) tsp 1½ with juice Crushed pineapple 1/2 cup d carrots 1 ½ cups Grate sauce Unsweetened apple ¾ cup Eggs 2 large Egg whites 2 large Vanilla extract 2 tsp NTS ICING INGREDIE 8 oz 13 gm 2 tsp Light cream cheese Stevia Vanilla INSTRUCTIONS Carrot Cake n with 3/4 ur a 10” tube pa dry 0 ˚F. Pam and flo heat oven to 35 , mix together all Pre medium bowl wheat flour. In a gether all wet cup whole bowl, mix to wet seperate medium redients, pour the ingredients. In a ...

Healthy Eating
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Lifestyle Coach Facilitation Guide: Core Session 4: Healthy Eating Table of Contents Background and Preparation Page Preparation Checklist Materials Required for Session 4 Before You Begin 2 Lifestyle Coach Brief Learning Objectives Session Overview Key Messages 4 Weigh-in Week in Review This Week 7 Classroom Presentation Part 1: Weekly Progress and Review Part 2: Healthy Ways to Eat Introduction The Way We Eat The Type of Food We Eat 10 Part 3: MyPlate MyPlate The Purpose of MyPlate MyPlate: Grains MyPlate: Vegetables MyPlate: Fruit MyPlate: Dairy MyPlate: Protein How Have You Been Eating? Eating More Like MyPlate 12 Part 4: Wrap Up and To-Do List To Do Next Week Closing 22 Notes and Homework 25 Follow Up Session 4: Healthy Eating Background and Preparation Preparation Checklist Materials These are the materials you will use during Session 4.  Participant handouts for Session 4...

Meal Planning Guide 1400 Calorie
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This Meal Planning Guide is based on the Exchange System, which groups foods into categories such as starches, fruits, vegetables, milk, meats and meat substitutes, and fats. One serving in a group is called an “exchange.” An exchange has about the same amount of carbohydrates, protein, fat, and calories as a serving of every other food in that same group. So, for example, you could swap one small apple for 3/4 cup of blueberries for one fruit serving. Sometimes nutrient figures may seem to vary a bit from list to list due to rounding-off, different serving sizes, or different methods of preparation. But if you eat the specified number of servings of each group, you can be comfortable that you’ll have a diet rich in nutrients and low in fat and calories. At first it appears a bit tedious measuring out an ounce of this and a cup of that, and with some foods it can be hard to measure or estimate an exact serving size. An inexpensive kitchen scale will come in handy. And these pictures will illustrate some helpful estimations:

Growing Naturals Rice Protein
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Axiom has launched a new marketing program to benefit clients incorporating Oryzatein into their products. Axiom is helping educate consumers that not all rice proteins are created equal and helps differentiate products utilizing Oryzatein.

Panduan penulisan Penerbitan Buku Teks - PDPT

Panduan Penulisan Buku Panduan ini merupakan petunjuk penulisan buku pelajaran (ilmiah populer) yang digunakan untuk menentukan kelayakan naskah bagi penerbit. Panduan ini membahas pengertian buku pelajaran & diktat, tujuan penulisan buku pelajaran, isi buku pelajaran, sampul buku, bagian pembuka, bagian utama dan bagian penutup serta ketentuan jumlah halaman. Buku Pelajaran (Text book) & Diktat Buku pelajaran adalah bahan/materi pelajaran yang dituangkan secara tertulis dalam bentuk buku dan digunakan sebagai bahan pelajaran (sumber informasi) sebuah mata kuliah bagi mahasiswa dan pengajar susuai dengan kebutuhan lapangan/industry dan tuntutan perkembangan teknologi dan atau kurikulum. Diktat adalah catatan tertulis suatu bidang studi yang disiapkan oleh guru/dosen untuk mempermudah pengayaan materi pelajaran atau bidang studi yang dibahas dalam proses pembelajaran (Ilvandri, 2011). Diktat yang baik merupakan draft buku ajar yang belum diterbitkan. Tujuan penulisan buku pelajaran a. Menyediakan buku susuai dengan kebutuhan mahasiswa, institusi dan lapangan/ industry serta serta tuntutan perkembangan teknologi atau kurikulum. b. Mendorong penulis/dosen untuk berkreasi dan kreatif membagikan ilmunya kepada masyarakat. c. Mendorong penulis untuk meng-update ilmunya sesuai dengan kriteria tuntutan buku layak terbit mencakup subdstansi, bahasa dan potensi pasar. d. Mendukung penulis untuk menerbitkan buku bila belum terbit. Isi Buku Pelajaran Isi buku pelajaran berupa teori, konsep, formula atau aturan terkini dilengkapi dengan contoh-contoh masalah atau studi kasus serta solusinya. Isi buku harus orsinil dengan merujuk dari berbagai sumber. Informasi tepat, dapat dipercaya dan dipertanggungjawabkan kepada pembaca dan semua pihak terkait. Isi tersusun dengan baik atau dengan alur informasi yang mudah dipahami. Buku pelajaran dan diktat yang baik memenuhi tiga aspek pendidikan yaitu ilmu pengetahuan (knowledge), keterampilan (skills) dan sikap atau perilaku (attitude). Aspek tersebut seperti yang dinyatakan oleh UNESCO (1994) yaitu...

Genetic, biochemical, and crystallographic characterization of Fhit ...

Proc. Natl. Acad. Sci. USA Vol. 95, pp. 5484–5489, May 1998 Biochemistry Genetic, biochemical, and crystallographic characterization of Fhit–substrate complexes as the active signaling form of Fhit HELEN C. PACE*, PRESTON N. GARRISON†, A NGELA K. ROBINSON†, L ARRY D. BARNES†, A LEXANDRA DRAGANESCU*, ¨ A NGELIKA ROSLER‡, G. MICHAEL BLACKBURN‡, ZURAB SIPRASHVILI*, CARLO M. CROCE*, KAY HUEBNER*, AND CHARLES BRENNER*§ *Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107; †Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78284; and ‡Department of Chemistry, Krebs Institute, University of Sheffield, Sheffield, S3 7HF England Communicated by Sidney Weinhouse, Jefferson Medical College, Philadelphia, PA, March 2, 1998 (received for review December 1, 1997) Because Fhit is encoded at the chromosome 3 fragile site, FRA3B (12), it had been suggested that cancer-associated deletions in the FHIT gene represent a ‘‘bystander effect’’ in which carcinogenesis was initiated by other unrelated genetic changes and that FHIT deletions resulted from genomic instability within already established tumors (13). However, if this were the case, reintroduction of FHIT cDNAs into tumors with FHIT deletions would not be expected to be consequential. On the contrary, stable reexpression of Fhit protein in gastric, lung, and kidney cancer cells with homozygous FHIT deletions suppressed the ability of those cells to form tumors in nude mice (14). It is important to emphasize that whereas FHIT passed this test as a tumor-suppressor gene, there are at least two scenarios in which genes with importance in carcinogenesis might fail this test. First, if loss of function of a gene were to create a mutator phenotype that led to further genetic lesions, reintroduction of that gene into tumor-derived cell lines might not reverse their tumor-forming ability. Second, in...

Tumor Suppressor Genes and Oncogenes - UCSF Biochemistry ...

• Describe the normal cellular functions of tumor suppressor genes and proto-oncogenes and explain their roles in cancer. • Describe Knudson’s two-hit hypothesis for the pathogenesis of retinoblastoma. • List the common clinical features, evolution and treatment for retinoblastoma. • Explain why loss-of-heterozygosity of a particular chromosome/chromosomal region in tumor DNA suggests the existence of a tumor suppressor gene in that region. • Explain why loss of either Rb or p16 drives a cell to proliferate. • Explain how the proteins encoded by the E6 and E7 genes of the oncogenic human papilloma viruses function to promote tumor formation. • Describe how the HER2/neu oncogene is activated in breast cancer. • Describe the normal function of Ras proteins and the molecular mechanism by which mutations in Ras genes lead to cancer. • Describe three pathways by which the cyclin D gene is activated in tumors. • Explain why activation of the Bcl-2 gene promotes cancer. • Describe the molecular events associated with different stages in the development of colon cancer. • Describe the six hallmark features of cancer cells and the molecular basis of each. KEY WORDS Adenomatous polyposis coli (APC) Bcl2 caretaker chromosomal inversion chromosome translocation cyclin D E6 protein E7 protein EMSY gain-of-function gene amplification HER2/neu...

How to find the most effective Elliptical machine Reviews Online

If you need for the solution to any running machine because your fitness machine, then you could get a beneficial best elliptical trainer for any work out plans you would finished in your abode. You are able to choose between a sluggish exercise plan to resistance training and additionally cardio workouts including a longterm preparation designed to protein shake most of the fatty acids at a distance. To keep a really good elliptical exercise machine usually is to have one that has a meter exhibit for this required info for instance the utilization required mentioned, the time period entire length together with the calorie consumption you felt the need shed. It can be better if a elliptical exercise machine will be definitely, powerful and offers somebody noise-free training. Parallel to in addition to superior to treadmill machines, you can buy a considerably fat-free body the usage of elliptical fitness machines.

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