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Organic & Biochemistry Laboratory Manual Chemistry 107L

Table of Contents Experiment Page Principles for Safety in the Chemical Laboratory.................................................................2 Laboratory Notebooks...........................................................................................................6 1,2,3,4... Counting Carbons to name that Organic Compound...........................................10 3-D Modeling of Organic Molecules..................................................................................20 Synthesis of Aspirin .............................................................................................................30 Characterization of Aspirin Synthesis Product Using TLC..................................................35 Characterization of Aspirin Synthesis Product Using NMR and IR....................................40 Saponification, a Sudsy Situation........................................................................................53 Identification of Carbohydrates .........................................................................................59 Testing For Vitamin C .......................................................................................................65 Isolating DNA in the Kitchen ............................................................................................72 Protein Identification Lab ..................................................................................................77 Using Excel to Graph Data.................................................................................................84 Protein Quantitation Using the Lowry Method................................................................. 93 Appendix I: Notes on Laboratory Glassware and Equipment ..........................................100 Appendix II. Notes on Significant Figures ........................................................................107

MEMBRANE AND CELLULAR BIOCHEMISTRY MBIO 3460 LAB ...

CONTENTS PAGE ______________________________________________________________________ EXPERIMENT SCHEDULE 3 GENERAL INSTRUCTIONS 4 LAB STANDARD OPERATIONS PROCEDURE 9 WHMIS 12 1 THE ISOLATION AND QUANTITATION OF UBIQUINONE Saponification Extraction and Evaporation TLC Scanning spectrophotometry Lab Report Do you know? Reagents and Functions 15 2 THE ISOLATION OF RED BLOOD CELL MEMBRANE PROTEINS Preparation of Red Blood Cell Membranes Protein Determination Polyacrylamide gel electrophoresis Lab Report Do you know? Reagents and Functions References 31 APPENDIX Excel Procedures 46 Pipetman Operation 49 Hitachi High Speed Himac Refrigerated Centrifuge 50 Sorvall Legend X1R Centrifuge Operation 52 Biochrom Ultraspec pro500 Operation 54 Biochrom Spec3100 Operation 55 SAMPLE LAB EXAM 56 3 MBOI 3460 Membrane and Cellular Biochemistry SCHEDULE 2013 All labs start at 2:30 pm unless otherwise stated. Section assignment is based on last name - see lab website for details. Week Section Room # Experiment/In-lab Assignment Date # 1 1 204 The isolation and quantitation of ubiquinone (1) Saponification Sept 13 2 2 204 The isolation and quantitation of ubiquinone (1) Saponification Sept 20 3 1 2 204 201 The isolation and quantitation of ubiquinone (2) TLC Sept 27 4 1 204 The isolation and quantitation of ubiquinone (3) Scanning spectrophotometry Oct 4 5 2 204 The isolation and quantitation of ubiquinone (3) Scanning spectrophotometry Oct 11 6 1 2 204 201 The isolation of red blood cell membrane proteins (1) Isolation and Bradford protein determination Oct 18 7 1 2 204 201 The isolation of red blood cell membrane proteins (2) Lowry protein determination and Polyacrylamide Gel Electrophoresis Oct 25 8 1&2 534 Nov 1 11 1&2 TBA EM Microscope Demonstration, 534 Buller, wait outside door at designated time for your group: groups 1 - 8 attend at 2:30 pm; groups 9-16 attend at 2:50 pm; groups 17-24 attend at 3:10 pm Lab exam Nov 22 MBIO 3460 Report Due Dates Week # Description Datea ....

Austrijska vojna obavestajna je zbog dvorske mrznje i samog cara Franje odvela princa sa suprugom na odstrel i dobila povod za pripremljeni rat

austrijska vojna obavestajna je zbog dvorske mrznje i samog cara Franje odvela princa sa suprugom na odstrel i dobila povod za pripremljeni rat,svi drugi prikazi su obicna glupost.Nego pustimo to,to je vec obelodanjeno ,KAKO bi bilo da se obelodani ime poljskog geliptera koji je unistio srbiju posle 44- ??nesmeseA ********* МАКС ВЕБЕР

Understand The Three Steps Of Credit Card Processing

MercuryPay offers affordable, secure and simple payment solutions for all types and sizes of businesses. We excel in multiple Merchant Accounts and Merchant Services which permits a company to accept credit and debit card payments.

Optimalon Software Released New Version of Online Panels Cutting Optimizer

1888PressRelease - New version of web-based application for panel-cutting optimization provides support of metric and imperial units, generates Excel report, imports/export panels and parts from Excel and export cutting layouts to AutoCAD DXF file.

BAB II PROFIL DEWAN PIMPINAN CABANG ( DPC ) PARTAI ...

Partai Demokrat didirikan atas inisiatif Susilo Bambang Yudhoyono yang terilhami oleh kekalahan terhormat Susilo Bambang Yudhoyono (selanjutnya disebut SBY) pada pemilihan calon wakil Presiden dalam Sidang MPR tahun 2001. Dari perolehan suara dalam pemilihan calon wakil Presiden (cawapres) dan hasil pooling public yang menunjukkan popularitas yang ada pada diri SBY, beberapa orang terpanggil untuk memikirkan bagaimana sosok SBY bisa dibawamenjadi Pemimpin bangasa dan bukan direncanakan menjadi wakil Presiden RI, tetapi menjadi Presiden RI untuk masa mendatang. Hasilnya adalah beberapa orang diantaranya, seperti Vence Rumangkang menyatakan dukungannya untuk mengusung SBY ke kursi Presiden, dan bahwa agar cita-cita tersebut dapat terlaksana, jalan satu-satunya adalah mendirikan partai politik.

BAB II DESKRIPSI PARTAI DEMOKRAT 2.1.Sejarah Pembentukan ...

DESKRIPSI PARTAI DEMOKRAT 2.1.Sejarah Pembentukan dan Berdirinya Partai Demokrat Partai Demokrat sebagai sebuah partai politik didirikan pada tanggal 9 September 2001. Berdirinya Partai Demokrat ini tidak terlepas dari inisiatif Susilo Bambang Yudhoyono. Susilo Bambang Yudhoyono merasa tergerag untuk membentuk sebuah partai baru ketika ia mengalami kekalahan terhotmatnya pada pemilihan calon wakil presiden dalam sidang MPR tahun 2001. Popularitas Susilo Bambang Yudhoyono pun mulai terlihat dari hasil perolehan suara calon wakil presiden tersebut. Hal tersebut pula lah yang kemudian menggundang kertetarikan beberapa orang untuk menjadikan Susilo Bambang Yudhoyono tidak hanya sebagai calon wakil presiden melainkan sebagai sosok pemimpin bangsa yang direncanakan mampu menjadi Presiden RI dimasa yang akan datang. Salah satu orang yang mempunyai pemikiran seperti itu adalah saudara Vance Rumangkang. Vance Rumangkang menyatakan dukungannya untuk mengusung Susilo Bambang Yudhoyono ke kursi Presiden RI, dan bahwa agar cita-cita tersebut bisa terlaksana, jalan satu-satunya adalah mendirikan partai politik. Dalam menindaklanjuti hal tersebut, perumusan konsep dasar dan platform partai pun kemudian dilakukan. Perumusan konsep dasar dan platform partai dilakukan oleh Tim Krisna Bambu Apus dan selanjutna mengenai teknis administrasi diselesaikan oleh tim yang dipimpin oleh saudara Vance Rumangkang. Selanjutnya, pada tanggal 12 Agustus 2001 pukul 17.00 diadakan rapat yang langsung dipimpin...

Springborn Staffing Skills Assessments Software Titles Tutorial Titles ...

Springborn Staffing Skills Assessments www.proveit2.com/springbornstaffing Software Titles ACCPAC Pro Series Adobe Acrobat 6.0 Standard Adobe Acrobat 7.0 Standard Adobe Acrobat 8 Standard Adobe Acrobat 9.0 Adobe Flash CS3 Professional Adobe Flex 3 Adobe Illustrator CS Adobe Illustrator CS2 Adobe Illustrator CS3 Adobe InDesign CS Adobe InDesign CS2 Adobe InDesign CS3 Adobe PageMaker 6.5 Adobe PageMaker 7.0 Adobe Photoshop 5.5 Adobe Photoshop CS Adobe Photoshop CS2 Adobe Photoshop CS3 Adobe Photoshop CS4 ADP – Payroll Corel Presentations 9.0 Corel Quattro Pro 9.0 Corel WordPerfect 9.0 – Normal User Corel WordPerfect 9.0 – Power User Corel WordPerfect 9.0 – Whole Test Desktop Publishing Theory Skills FileMaker Pro 6 JD Edwards Legal MacPac 2000 Lotus 1–2–3 Millennium Lotus Freelance Graphics Millennium Lotus Notes 4.5 for Users Lotus Notes 4.6 Lotus Notes 5.0 for Users Lotus Notes 6.5 for Users Lotus Notes 8.5 for Users Lotus Notes R5 Lotus Word Pro Millennium Macintosh Basics OS 9 MAS 90 – Bookkeeping Microsoft Access 2000 Microsoft Access 2002 Microsoft Access 2003 Microsoft Access 2007 Microsoft Microsoft Microsoft Microsoft Microsoft Microsoft Microsoft Microsoft Microsoft Microsoft Microsoft Access 2000 Tutorial Access 2002 Tutorial Access 2003 Tutorial Access 2007 Tutorial Excel 2000 Tutorial Excel 2002 Tutorial Excel 2003 Tutorial Excel 2007 Tutorial Internet Explorer 5.0 Tutorial Internet Explorer 8.0 Tutorial Office 2000 Integration Tutorial ACT! 2000 for Users Advanced Spelling Analytical Skills Arithmetic Audio Typing [5 Minutes] Basic Arithmetic Basic Numeric Conversion Basic Office Skills Basic Office Skills [No Math] Basic Office Skills [No Verbal] Basic Reading Comprehension Excel 2000 – Normal User Excel 2000 – Power User Excel 2000 – Whole Test Excel 2002 – Normal User Excel 2002 – Power User Excel 2002 – Whole Test Excel 2003 – Normal User Excel 2003 – Power User Excel 2003 – Whole Test Excel 2007 – Normal User Excel 2007 – Power User Excel 2007 – Whole Test FrontPage 2003 Internet Explorer 5.0 Internet Explorer 6.0 Internet Explorer 8 Office 2000 Integration Office 2003 Integration Office XP Integration Outlook 2000 Outlook 2002 Outlook 2003 Outlook 2007 PowerPoint 2000 PowerPoint 2002 – Normal PowerPoint 2002 – Power PowerPoint 2002 – Whole PowerPoint 2003 – Normal PowerPoint 2003 – Power PowerPoint 2003 – Whole PowerPoint 2007 – Normal PowerPoint 2007 – Power PowerPoint 2007 – Whole

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For those who want to excel online payment for school in Ghaziabad

Even in Singapore, where 44% of the population has access to the Internet, only 16% of Internet users have conducted purchase transactions online (Kuo et. al, 2001). In the US, where almost two-thirds have access to the Internet, over 50% have transacted online (Cole et al, 2000)

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